Emma Ribom's sluggish first leg seemed set to cost Sweden the title as she handed over to Frida Karlsson a huge 36 seconds behind Norway.
However, that advantage remained steady until Ebba Andersson handed over to Jonna Sundling for the final leg.
The 30-year-old swiftly closed the gap on Norway's Kristin Austgulen Fosnaes and they entered the stadium side-by-side.
But the Norwegian stood no chance against Sundling, who stayed in control all the way to the finish line and has now won three golds at this year's worlds, including two in sprint.
Andersson has also taken her personal gold tally to three.
Sweden have taken all five golds so far in the women's cross-country events at Trondheim 2025 and a win in the 50km freestyle on Sunday will complete a clean sweep.
In total, the Swedish team have picked up six golds and four bronzes, leaving them second in the medal table behind Norway.