She will work the bases in both games of Saturday's doubleheader and will be stationed behind home plate on Sunday, MLB said.
Pawol, who has umpired in the minors previously, was the first woman to umpire in a Spring Training game for 17 years during a meeting between the Houston Astros and Washington Nationals in 2024.
The 48-year-old New Jersey native played college softball and has been a minor league umpire since 2016.
The barrier-breaking news comes 10 years after the National Football League hired its first full-time female official, Sarah Thomas, who would go on to become the first woman to officiate a Super Bowl in 2021.
The NBA was the first of the "big four" men's professional leagues in North America to add women to its full-time officiating staff, hiring Violet Palmer and Dee Kantner in 1997.